Main Characters
- Scooby-Doo - Frank Welker
- Shaggy Rogers - Casey Kasem
- Fred Jones - Frank Welker
- Daphne Blake - Grey Delisle
- Velma Dinkley - Mindy Cohn
Recurring Characters
- Melbourne O'Reilly - Steven Jay Blum
- Elliot Binber - Kimberly Brooks
- Mr. B - Jeff Bennett
- Chrissie - Grey Delisle
- Secret Six' puppies - Jennifer Hale, Colleen O'Shaughnessey, Dee Bradley Baker, Grey Delisle, Frank Welker
- Gibby Norton
- Burr Batson - James Arnold Taylor
- The Hex Girls - Jennifer Hale, Jane Weilden, Kimberly Brooks
- J.J. Hakimoto - Brian Tochi
Guest Stars
Villains
- Snow Creature
- Dinosaur Spirit
- Space Ape
- The Ghost of Jed and Caleb Leland
- The Possessed Mystery Machine
- The Ghost of Rufus Raucous
- Roller Ghoster
- The Jungle Demons
- Motoshandu Sea Monster
- The Headless Snowman
- The Living Toys
- Faceless Phantom
- Zombie Gladiator and the Emperor
- Baseball Spectre
- The 30ft. Shaggy Monster
- The Mummy
- Giant Worm
- Haunted House and The Robot Drone
- Vampire Fortesque
- The Scarecrows and the Ghost of the Hank Banning
- Cat Creature
- San Fran Psycho
- Invisible Madman
- Scooby Snack Creature
- Black Dragon
- Finned Fiend
- Centaur
- Creepy Keeper
- Robot Bandits
- Titanic Twist
- Demon Mystery Inc. Imposters
- Gargoyle
- Demon Farmer
- Frozen Fiend
- Metallic Clown
- Toxic Terror
- Chinese Dragon
Other Characters
- Zelig
- Celia Clyde
- Danica LeBlake
- Brook Bjork
- Rutie Banez
- Shawn and Shauna
- Alex, Jake and Ernie
- Louie Hong Fa
- Rufas Rafus
- Chris and Terry
- [[
- [[
- [[
- Keith Dale
- Shane Flinty
- Ewan McEwan